Cilia
and flagella are tiny waving filaments with important functions in humans, such
as clearing our airways. A 3D electron microscopy study of the normal (WT) and
mutant varieties of flagella has revealed new details of the structure, protein
composition, and connections between neighboring components formed by a crucial
structural complex of this biological nanomachine,
the "nexin-dynein
regulatory complex" (N-DRC), which is shown in color on the left. In the poorly
moving mutants, this structure is damaged or almost missing. This opens the way
for building a new mechanical model of this device, and how it functions, along
with mechanical testing of individual cilia and flagella, normal and mutant, in
the Brandeis multi-mode optical microscopy laboratory.